Application of Biorefinery Concept to the Production of Bromelain, Ethanol, and Xylitol from Pineapple Plant Waste

The massive potential of pineapple fruit production can produce a sizable amount waste, around 75% (w/w) weight, contributing to global environmental problems. For this reason, biorefinery techniques are urgently needed convert plantation waste into high-value-added bioproducts including bromelain, various sugars, xylooligoscharide, xylitol, and ethanol. purpose study was examine the effectiveness converting In study, activity bromelain enzyme tested in each part plant waste. configuration hydrolysis fermentation processes used make ethanol xylitol from rest extraction also investigated. Bromelain is proteolytic found plants be isolated every Enzyme under several conditions, such as crude extract, pure dried has been studied determine best conditions for downstream process enzyme’s future. purification involved utilization precipitation method followed by dialysis, whereas drying employed freeze-drying method. specific shown highest stem, observed crude-extract (1.45 ± 0.06 CDU/mg), purified-extract (10.38 dried-extract (12.05 0.43 CDU/mg) conditions. Using stem extract lignocellulosic which made up 39.47% starch, 19.96% hemicellulose, 36.44% cellulose, 6.05% lignin. high content hemicellulose feedstock fermentation. were carried out using two methods: separate methods (SHF) semi-simultaneous saccharification (semi-SSF). As result, semi-SSF produced with higher titer yield (22.12 0.05 g/L 0.44 0.00 g/g, respectively). However, insignificant, regardless whether it obtained SHF or semi-SSF.